2. Results The purified high producing collagenase was isolated.
结果获得了高产量、高比活性的胶原酶。
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3. Objectives:To study collagenase activity in degenerated cervical discs.
目的：研究退变颈椎间盘中的胶原酶的活性变化。
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4. Substrate degradation by type IV collagenase was measured by zymography.
采用酶谱方法检测IV型胶原酶对底物的降解。
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5. Hepatocytes were isolated by the digitonin-collagenase perfusion technique.
肝细胞通过毛地黄皂苷-胶原酶灌流技术分离。
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6. Objective To isolate and purify collagenase with high quantity and activity.
目的分离获得高产量、高比活性的胶原酶。
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7. OBJECTIVE To prepare the collagenase immunizing conjugation product for injection.
目的：制备注射用胶原蛋白酶免疫结合物。
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8. Objective To discuss why the Collagenase cure the intervertebral disc disease but not effective .
目的探讨胶原酶对部分椎间盘突出病人治疗无效的原因。
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9. Objective: To study the values of treating the cervical disc herniation by collagenase under ct guiding.
前言：目的：研究CT引导下胶原酶注射颈椎间盘溶解术治疗颈椎间盘突出症的价值。
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10. Collagenase is one kind of enzymes that can degrade native helical collagen fibrils or glutin but not degrade others proteins.
胶原酶是一种特异降解胶原螺旋区或明胶，而不作用于其它蛋白质底物的酶类。
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11. Objective To study the clinical significance of the combined target-injection of collagenase by ct guided for local chemonucleolysis.
目的探讨CT引导联合靶注射胶原酶定点髓核溶解术的临床应用价值。
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12. Objective To assess the therapeutic effect of oxygen-ozone combined collagenase injection for the treatment of lumbar disc herniation.
目的研究臭氧髓核氧化术结合胶原酶溶解术治疗腰椎间盘突出症的临床疗效。
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13. The experimental groups were injected collagenase under sheaths, and the control groups were injected physiological saline respectively.
实验趾和对照趾腱鞘内分别注入胶原酶和生理盐水。
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14. MethodsPrimary human insulinoma cells were isolated by using collagenase type p, and then purified by repeatedly selective cell culture.
方法原代分离人胰岛素瘤细胞，经多次筛选培养进行纯化。
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15. Conclusion SCA can induce endothelial cell apoptosis and inhibit VEGF expression as well as type iv collagenase activity in cancer cells.
结论sca可诱导内皮细胞凋亡，并抑制癌细胞VEGF表达及IV型胶原酶的降解活性。
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16. Objective To improve the puncture point to lateral recess and select the indication of collagenase injection for lumber intervertebral disc.
目的改进侧隐窝穿刺的体表定点，筛选胶原酶注射的适应证，提高疗效。
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17. AIM: to observe effect of collagenase injected by different method on lumbar disc protrusion and imaging changes and to analyze its mechanism.
目的：观察胶原酶不同注射方法对腰椎间盘突出症的康复效果及影像学变化，分析其治疗机制。
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18. In experiment, the rat models of AICH was created by collagenase with heparin, the brain water content and plasma biochemical index were observed.
动物实验方面，采用胶原酶合肝素复制大鼠脑出血模型。测定大鼠脑含水量、血浆生化等指标。
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19. Objective: To explore the effects of nursing interference on the injection of collagenase in the treatment of lumbar intervertebral disc protrusion.
目的：探讨康复护理对胶原酶溶解术治疗腰椎间盘突出症的疗效。
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20. Objective To investigate systematical changes of hemorheology on the model of cerebral hemorrhage induced by type I collagenase and heparin in rats.
目的系统研究I型胶原酶-肝素诱导的大鼠脑出血后血液流变学变化情况。
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21. Objective: To evaluate the clinical efficacy of the target-injection of collagenase under ct intervention for the treatment of lumbar disc herniation.
目的：探讨CT介入靶位注射胶原酶治疗腰椎间盘突出症的临床应用价值。
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22. Objective the purpose of this study was to evaluate the effect of degradation of human root dentin collagen treated with collagenase after demineralization.
目的探讨胶原酶对脱矿后的人牙根面牙本质胶原蛋白的降解作用。
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23. CONCLUSIONS: tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells.
结论：在对根、冠髓细胞的原代培养中，组织块法优于组织块酶解法。
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24. Objective:To investigate the clinical significance of the target-injection of collagenase for the treatment of recurrent postoperative lumbar disc herniation.
目的：探讨靶位注射胶原酶治疗腰椎间盘突出术后复发的临床意义。
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25. Conclusion:The target-injection of collagenase for intervertebral disk chemolysis may be complementary therapy for recurrent postoperative lumbar disc herniation.
结论：靶位注射胶原酶溶盘术可与手术互补，适用于腰椎间盘突出术后复发的治疗。
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26. Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
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27. Objective To observe the relationship between morphological mutation of rabbit cornea after cornea alkali burn and the variation of collagenase content in lacrima.
目的观察兔角膜碱烧伤后形态学变化与泪液中胶原酶含量变化间的关系。
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28. Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
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29. Methods: Based on the rat's hypertension model made by clipping both kidneys, we made a CCS model by infusing collagenase and heparin into the rat's caudate nucleus.
30. Methods: Based on the rat's hypertension model made by clipping both kidneys, we made a CCS model by infusing collagenase and heparin into the rat's caudate nucleus.
